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| Registros recuperados: 57 | |
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| Antunes,Alexsander Z.. |
| Diurnal variation in detection can influence bird census results. I measured variation in daily and seasonal detections of birds in a forest fragment at Barreiro Rico ranch, Anhembi, state of São Paulo. Birds were recorded on transects of unlimited distance, sampled one day per month, dawn to dusk, between December 2000 and March 2001 (rainy season) and between May and August 2001 (dry season). Significant daily variation in detection occurred during the rainy season, with most detections between dawn and 9 h. In the dry season detections declined for total numbers of individuals and species and most detections occurred during late morning and mid afternoon. Detection patterns might reflect seasonal variation in bird behavior rather than seasonal variation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atlantic Forest; Census; Detection; Seasonal variation; Transects. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752008000200011 |
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| Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Berthe, Franck. |
| Bonamiosis was first reported in association with mass mortality of flat oysters, Ostrea edulis, in June 1979 in L'Ile Tudy, Brittany. The disease rapidly spread to all of the oyster farming areas in France but also in other European countries. The French flat oyster production, which already suffered from another protozoan disease, marteiliosis, decreased from 5500 mt in 1979 to less than 2000 mt after 1980. In 2001, about 350 French farms sell 1650 mt of flat oysters. This production mainly relies on natural spat collection which specially occurs in Quiberon bay. One third of this spat is transferred from South to North Brittany for further growth. Quiberon bay constitutes an interesting site regarding the surveillance of bonamiosis because of the... |
| Tipo: Text |
Palavras-chave: Detection; Bonamia ostreae; Protozoan; Diseases; Pathology; Ostrea edulis; Oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3314.pdf |
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| Barragan,Carlos Eduardo; Guzmán-Barney,Mónica. |
| Potato yellow vein virus (PYVV) expresses yellowing symptoms on plants and it is transmitted by the whitefly vector Trialeurodes vaporariorum Westwood. No molecular viral detection from the vector extracts has been reported to date. The objectives of this study were I) amplification of the PYVV major coat protein (CP) gene from viruliferous vector extracts by RT-PCR using specific primers, II) cloning and III) sequencing and sequences analysis. Some Colombian Solanum phureja (Jus et Buk) field potato plants expressing yellowing symptoms and confirmed PYVV positive by RT-PCR were used as virus donor plants for feeding whiteflies. Viruliferous vectors were obtained by depositing aviruliferous whiteflies on symptomatic leaves and kept in a muslin... |
| Tipo: Journal article |
Palavras-chave: PYVV; RNA; Whitefly; Detection; Vector. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000300002 |
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| Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan. |
| Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1... |
| Tipo: Text |
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf |
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| Benetti,TM; Abrahão,WM; Ferro,ID; Macedo,REF; Oliveira,TCRM. |
| ABSTRACT In 2006, the International Organization for Standardization (ISO) published two standard protocols for the detection and enumeration of Campylobacter spp. in foods: the ISO 10272-2(direct method) and the ISO 10272-1 (enrichment method). The aim of this study was to compare the efficiency of these two methods in the detection of Campylobacter spp. and propose a modification in the enrichment method. Compared with the enrichment method, the direct method yielded a higher number of positive results for Campylobacter spp. and, consequently, presented higher sensitivity percentage. The isolation of Campylobacter spp. was more difficult when 10 mL of rinse was used in the enrichment method, which is currently recommended by the ISO protocol. Therefore,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter spp.; Detection; ISO 10272; Refrigerated chicken carcass. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100023 |
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| Bosch, Albert; Gkogka, Elissavet; Le Guyader, Soizick; Loisy-hamon, Fabienne; Lee, Alvin; Van Lieshout, Lilou; Marthi, Balkumar; Myrmel, Mette; Sansom, Annette; Schultz, Anna Charlotte; Winkler, Anett; Zuber, Sophie; Phister, Trevor. |
| In a recent report by risk assessment experts on the identification of food safety priorities using the Delphi technique, foodborne viruses were recognized among the top rated food safety priorities and have become a greater concern to the food industry over the past few years. Food safety experts agreed that control measures for viruses throughout the food chain are required. However, much still needs to be understood with regard to the effectiveness of these controls and how to properly validate their performance, whether it is personal hygiene of food handlers or the effects of processing of at risk foods or the interpretation and action required on positive virus test result. This manuscript provides a description of foodborne viruses and their... |
| Tipo: Text |
Palavras-chave: Virus; Detection; Risk assessment; Food; Processing technologies. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00443/55507/57057.pdf |
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| Bosch, Albert; Sanchez, Gloria; Abbaszadegan, Morteza; Carducci, Annalaura; Guix, Susana; Le Guyader, Soizick; Netshikweta, Rembuluwani; Pinto, Rosa M.; Van Der Poel, Wim H. M.; Rutjes, Saskia; Sano, Daisuke; Taylor, Maureen B.; Van Zyl, Walda B.; Rodriguez-lazaro, David; Kovac, Katarina; Sellwood, Jane. |
| Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses... |
| Tipo: Text |
Palavras-chave: Enteric viruses; Gastroenteritis; Hepatitis; Detection; Concentration. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14191/11465.pdf |
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| Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa. |
| The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Detection; PCR; Pork; Salmonella; Swine. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013 |
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| Chao,T.P.; Sperandio,E.F.; Ostolin,T.L.V.P.; Almeida,V.R.; Romiti,M.; Gagliardi,A.R.T.; Arantes,R.L.; Dourado,V.Z.. |
| Spirometry has been used as the main strategy for assessing ventilatory changes related to occupational exposure to particulate matter (OEPM). However, in some cases, as one of its limitations, it may not be sensitive enough to show abnormalities before extensive damage, as seen in restrictive lung diseases. Therefore, we hypothesized that cardiopulmonary exercise testing (CPET) may be better than spirometry to detect early ventilatory impairment caused by OEPM. We selected 135 male workers with at least one year of exposure. After collection of self-reported socioeconomic status, educational level, and cardiovascular risk data, participants underwent spirometry, CPET, body composition assessment (bioelectrical impedance), and triaxial accelerometry (for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Occupational exposure to particulate matter; Cardiopulmonary exercise testing; Detection; Spirometry; Ventilatory impairment. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500612 |
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| Colombo,Tatiana Elias; Terzian,Ana Carolina Bernardes; Araújo Júnior,João Pessoa; Parreira,Ricardo; Cabrera,Eliana Márcia Sotello; Santos,Izalco Nuremberg Penha dos; Reis,Andréia Francesli Negri; Costa,Fabiana Rodrigues; Cruz,Lilian Elisa Arão Antônio; Rombola,Patrícia Lopes; Nogueira,Maurício Lacerda. |
| ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison;... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zika virus; Detection; Sensitivity; Specificity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100144 |
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| Daste, S. |
| The "CRABE" method is a crack detection technique based on the use of low electromagnetic frequencies. This (patented) method relies on a global injection of current within the structure under inspection. The presence of a crack shall cause a disruption in the current flow, and consequently of the magnetic field off the surface in the vicinity of a defect. It is this magnetic signature which is detected by the magnetic sensor used with "CRABE". The major advantage of this process, developed by TRAVOCEAN and the LETI Division of CEA with the help of IFREMER, appears clearly: No preliminary cleaning of the welds to be inspected is required, since no contact is necessary. Similarly, it shall be possible to inspect the whole of structures to a total cost all... |
| Tipo: Text |
Palavras-chave: Offshore structures; Fouling; Underwater exploration; Steel structures; Sensors; Cracks; Detection; Unmanned vehicles; Marine technology. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/1990/acte-1161.pdf |
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| David, R.; Treguier, Cathy; Montagnani, Caroline; Belliard, Corinne; Levy, Peva; Nedelec, Georges; Joufoques, Vaiana; Remoissenet, G.; Gueguen, Yannick; Cochennec-laureau, Nathalie. |
| Tipo: Text |
Palavras-chave: Betanodavirus; Detection; French Polynesia; Genotype; Platax orbicularis. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00003/11380/7972.pdf |
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| FAJARDO,THOR V. M.; NISHIJIMA,MARTA; BUSO,JOSÉ A.; TORRES,ANTÔNIO C.; ÁVILA,ANTÔNIO C.; RESENDE,RENATO O.. |
| Garlic viruses often occur in complex infections in nature. In this study, a garlic virus complex, collected in fields in Brazil, was purified. RT-PCR was performed using specific primers designed from the consensus regions of the coat protein genes of Onion yellow dwarf virus, a garlic strain (OYDV-G) and Leek yellow stripe virus (LYSV). cDNA of Garlic common latent virus (GCLV) was synthesized using oligo-dT and random primers. By these procedures individual garlic virus genomes were isolated and sequenced. The nucleotide sequence analysis associated with serological data reveals the presence of two Potyvirus OYDV-G and LYSV, and GCLV, a Carlavirus, simultaneously infecting garlic plants. Deduced amino acid sequences of the Brazilian isolates were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: OYDV-G; LYSV; GCLV; Detection; Coat protein; PCR; Probes; Epidemiology. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300007 |
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| Registros recuperados: 57 | |
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